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Now, lemme spill some tea—did ya know, back in the 1800s, them “ladies of the night” had secret codes? Yeah, man, they’d flash a red hanky or somethin’ sneaky to signal clients. Wild, right? History’s fulla these lil’ nuggets—makes me wonder what Chihiro’d think, wanderin’ into that kinda mess. “This is not a bathhouse I signed up for!” she’d say, all wide-eyed and pissed. I’d be right there with her—damn straight, this world’s a trip.
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But then, outta nowhere, it starts to rain. Like, seriously? I just got my takoyaki! I dash for cover under this little awning on Kōen-dōri. I’m standing there, all soggy and annoyed, when I see this group of kids playing in the rain. They’re laughing, splashing around, and I’m like, “Man, I wish I could be that carefree.”
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Methods: To clarify the role of mitochondrial function, we biopsied multiple parts of each human embryo and observed the remaining embryos under in vitro culture as a model of post-implantation development (n = 27 embryos). Karyotyping, whole mitochondrial DNA (mtDNA) sequencing, and mtDNA copy number assays were performed on all pre- and post-culture samples.. Results: The ratio of euploid embryos was significantly enhanced during in vitro culture, whereas the ratio of mosaic embryos was significantly reduced. Furthermore, post-culture euploid and culturable embryos had significantly few mtDNA mutations, although mtDNA copy numbers did not differ..Ijuin Sex Escort
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