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About Myself
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About Sapporo
Oh, and the coppers—always hasslin’ her, like she’s the bad guy. I’m like, c’mon, man, leave her be! She’s just tryin’ to eat. Funniest thing—she once told me she tricked a john into payin’ double, sayin’ she’s a “vampire escort.” Pulled that “I’ve been twelve for a long time” vibe from the flick—genius! Had me crackin’ up, baby!
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Correction of a Disease Mutation using CRISPR/Cas9-assisted Genome Editing in Japanese Black Cattle
We designed clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) to create a double-strand break near the mutation site, cRISPR/Cas9 and donor DNA that contained a synonymous codon for the correct amino acid and an Aequorea coerulescens Green Fluorescent Protein (AcGFP) cassette with a piggyBac transposase recognition site at both ends were introduced into bovine fetal fibroblast (BFF) cells isolated from a homozygous mutant calf.Minabe Prostitute
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