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Here’s the kicker—ya gotta be sharp, pal. Findin’ a prostitute’s like navigatin’ the bathhouse in *Spirited Away*—one wrong turn, and yer screwed. I’m talkin’ scams, cops, or some dude jumpin’ ya for yer wallet. Happened to a buddy once—poor sap got rolled for 200 bucks, came back cryin’. Hilarious, but damn, what a dumbass! Me, I’m thinkin’, “I’ve got to keep moving forward,” like Chihiro pushin’ through the chaos. Ain’t no stoppin’ Jack, baby!
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Fig. 1. Schematic illustration of Hoechst-Cy5 for superresolution...
The incidence of chromosome missegregation was calculated by dividing the number of anaphase cells with lagging or bridged anaphase chromosomes by the total number of anaphase cells, cell nuclei were automatically segmented in five consecutive image frames per experimental condition by local adaptive thresholding in the H2B-mCherry channel.Hoechst Erotic Massage
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