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About Myself
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Oh, and the smells—god forbid! Stale perfume, cigarette smoke—my nose would revolt! I’d be rantin’, “Can’t you Febreeze this joint?” Total “Dogville” moment—Grace puttin’ up with filth, me whinin’ about it. I’d probly tip too much outta guilt—neurotic, right? “Here’s an extra twenty—sorry for existin’!” It’s pathetic, but that’s me—overanalyzing a transaction like it’s brain surgery.
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So, I’m strolling down the Hoofdstraat, right? Just minding my own biz, when I spot this cute little café. It’s called “De Koffieboon.” I mean, who doesn’t love coffee? I grab a cappuccino, and it’s like a hug in a mug. I’m feeling all warm and fuzzy, ya know?
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Despite the fact that many of these techniques are currently used in AIE diagnostic and research laboratories, the methodology to detect cell-surface antibodies is not widely standardized. In this article we review the advantages and pitfalls of three different techniques for antibody detection: (1) IHC/IIF on adult rat/primate brain slices; (2) ICC on living cultured rat hippocampal neurons; and (3) CBAs for neuronal membrane proteins (for an overview see Table 1), in addition we evaluate the use of immunoprecipitation and mass spectrometry analysis for the identification of novel cell-surface antigens.Coevorden Prostitute
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